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Anti-Collagen II抗體售后服務

時間:2014-3-25閱讀:243
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Mouse Anti-Collagen II抗體售后服務

FROM: ABCAM
Catalog  Number : BYK-
0709R       
Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Anti-Collagen II抗體售后服務Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues.

Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Specificity :   

  • Anti-Collagen II is a mouse monoclonal antibody unconjugated                
  • specific for MUSK of hu, mo, rat, Rb, cow, dog, chk, Gpig, pig           
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • mol wt:  117/163kDa

Anti-Collagen II抗體售后服務Application :  

  • Western blotting 1:100-500                
  • Immunohistochemistry 1:100-500                 
  • ELISA 1:500-1000                  
  • Optimal working dilutions must be determined by the end user.

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4

Anti-Collagen II抗體售后服務Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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