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Anti-Smoothened/SMO抗體

時間:2014-3-27閱讀:340
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Rabbit  Anti-Smoothened/SMO抗體

FROM:ABCAM
Catalog Number : BYK -2801R       
Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Anti-Smoothened/SMO抗體Background: Smoothened (SMOH), a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein (PTCH) to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas.

SMO expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Specificity :   

  • Anti-Smoothened/SMO is a rabbit polyclonal antibody unconjugated                
  • specific for Smoothened/SMO of hu, mo, rat, pig, Rb, hrs, chk, dog, cow         
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • mol wt:  86kDa

Anti-Smoothened/SMO抗體Application :  

  • Western blotting 1:100-500                
  • Immunohistochemistry 1:100-500
  • IF=1:100-500  IP=1:20-100                 
  • ELISA 1:500-1000
  •  IHC-P=1:100-500  IHC-F=1:100-500                 
  • Optimal working dilutions must  be determined by the end user.

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

Anti-Smoothened/SMO抗體Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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