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產(chǎn)品名稱:DAPI溶液(即用型10μg/ml )操作步驟
英文名字:DAPI solution (ready use10μg/ml)
產(chǎn)品描述
DAPI溶液(即用型10 μg/ml )即 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI),中文名為4’,6-二脒基-2-苯基吲哚。本產(chǎn)品是一種可以穿透細(xì)胞膜的DNA鏈藍(lán)色熒光染料,DAPI染色常用于細(xì)胞凋亡檢測(cè),染色后用熒光顯微鏡觀察或流式細(xì)胞儀檢測(cè)。DAPI也常用于普通的細(xì)胞核染色以及某些特定情況下的雙鏈DNA染色。
產(chǎn)品優(yōu)勢(shì)
本產(chǎn)品為DAPI水溶液,純度≥90%,為即用型工作液,可以直接用于固定細(xì)胞或組織的細(xì)胞核染色。
儲(chǔ)存條件
−20℃避光保存。
DAPI溶液(即用型10μg/ml )操作步驟注意事項(xiàng)?
DAPI對(duì)人體有一定刺激性,請(qǐng)注意適當(dāng)防護(hù)。
熒光染料都存在淬滅的問(wèn)題,建議染色后盡量當(dāng)天完成檢測(cè)。
為減緩熒光淬滅可以使用抗熒光淬滅封片液。
為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
DAPI溶液(即用型10μg/ml )操作步驟儲(chǔ)存條件:
大部分的試劑產(chǎn)品是比較穩(wěn)定的,只要您嚴(yán)格按照產(chǎn)品包裝或者說(shuō)明書上的儲(chǔ)存條件進(jìn)行儲(chǔ)存,*可以保證其在有效期內(nèi)的正常使用。一般常見儲(chǔ)存條件有零下XX攝氏度,2-8攝氏度,常溫等。特別提醒,對(duì)于某些酶類制品,如果儲(chǔ)存條件是2-8攝氏度,不要儲(chǔ)存在零下,否則容易失活,對(duì)于某些需要嚴(yán)格避光,防潮,充氮?dú)獗Wo(hù)的,接觸空氣或者過(guò)分暴露非常容易氧化或者受潮變質(zhì)。
低溫儲(chǔ)存產(chǎn)品,運(yùn)輸途中冷藏問(wèn)題:
對(duì)于需要低溫儲(chǔ)存的產(chǎn)品,我們?cè)谶\(yùn)輸時(shí)候都會(huì)采用冷藏包裝,但是部分由于外界氣溫過(guò)高,運(yùn)輸時(shí)間較長(zhǎng),對(duì)于普通冷藏包裝,途中可能會(huì)溫度升高,這些都是沒(méi)有問(wèn)題的,因?yàn)檫@種普通冷藏只適用于那些短期內(nèi)(2周)高溫依然穩(wěn)定的產(chǎn)品,建議低溫的儲(chǔ)存條件,只是長(zhǎng)期儲(chǔ)存時(shí)候的條件,您可以放心使用。對(duì)于某些特殊的產(chǎn)品,如抗體,我們會(huì)采用干冰冷藏包裝,確保全程低溫。公司都對(duì)產(chǎn)品性狀進(jìn)行了嚴(yán)格的加速穩(wěn)定測(cè)試,行業(yè)經(jīng)驗(yàn)豐富。
DAPI溶液(即用型10μg/ml )操作步驟分裝及溶液配制:
您收到貨物后不要自己進(jìn)行分包,因?yàn)榉职h(huán)境、包裝材料等因素可能導(dǎo)致分包后的產(chǎn)品變質(zhì);如您有特殊包裝要求,請(qǐng)?jiān)谟嗁?gòu)時(shí)候與我們客服代表闡明,當(dāng)然價(jià)格會(huì)做適當(dāng)調(diào)整。對(duì)于開蓋后,長(zhǎng)期未使用的,請(qǐng)務(wù)必重新密封好,建議Parafilm封口膜,并按照相應(yīng)儲(chǔ)存條件使用。如果放置時(shí)間過(guò)長(zhǎng),超過(guò)產(chǎn)品有效期,建議您重新購(gòu)買,以免影響實(shí)驗(yàn)質(zhì)量。
DAPI溶液(即用型10μg/ml )操作步驟產(chǎn)品應(yīng)用:
對(duì)于一些相對(duì)特殊的產(chǎn)品,我們會(huì)提供產(chǎn)品說(shuō)明書,里面會(huì)對(duì)產(chǎn)品溶解性,及我們所能了解的產(chǎn)品應(yīng)用信息,實(shí)驗(yàn)方案進(jìn)行描述,您可以作為參考。對(duì)于常見試劑,因?yàn)閼?yīng)用廣泛,一般不提供說(shuō)明書。如果您在實(shí)驗(yàn)過(guò)程中遇到了問(wèn)題,歡迎與我們討論,我們將盡力幫您解決。
DAPI溶液(即用型10μg/ml )操作步驟儲(chǔ)存條件:避光、干燥陰涼處封閉貯存,嚴(yán)禁與有毒、有害物品混放、混運(yùn)。本品為非危險(xiǎn) 產(chǎn)品可按一般化學(xué)品運(yùn)輸,輕搬動(dòng)輕放,防止日曬、雨淋!受熱、受潮、受光后易喪失活力,保存期短,因此貯存和運(yùn)輸條件比較苛刻。
CFN99988 http://www.chemfaces。。com/natural/Saikosaponin-C-CFN99988.html C48H72O17 927.14 20736-08-7 Saikosaponin C Triterpenoids >=98% 20mg
CFN99992 http://www.chemfaces。。com/natural/Indigo-CFN99992.html C16H10N2O2 262.26 482-89-3 Indigo Alkaloids >=98% 20mg
CFN99995 http://www.chemfaces。。com/natural/2-3-5-4-Tetrahydroxyl-diphenylethylene-2-O-glucoside-CFN99995.html C20H22O9 406.39 82373-94-2 2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside Phenols >=98% 20mg
CFN98211 http://www.chemfaces。。com/natural/Guaijaverin-CFN98211.html C20H18O11 434.4 22255-13-6 Guaijaverin Flavonoids >=98% 10mg
CFN98103 http://www.chemfaces。。com/natural/Jujuboside-D-CFN98103.html C58H94O26 1207.35 194851-84-8 Jujuboside D Triterpenoids >=98% 20mg
CFN98108 http://www.chemfaces。。com/natural/Jatrorrhizine-Hydrochloride-CFN98108.html C20H20ClNO4 373.83 6681-15-8 Jatrorrhizine Hydrochloride Alkaloids >=98% 20mg
CFN98110 http://www.chemfaces。。com/natural/Homoharringtonine-CFN98110.html C29H39NO9 545.61 26833-87-4 Homoharringtonine Alkaloids >=98% 20mg
CFN98111 http://www.chemfaces。。com/natural/Gramine-CFN98111.html C11H14N2 174.25 87-52-5 Gramine Alkaloids >=98% 20mg
CFN98113 http://www.chemfaces。。com/natural/Punicalin-CFN98113.html C34H22O22 782.52 65995-64-4 Punicalin Phenols >=98% 10mg
this entry.
Ectrodactyly, ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3) [MIM:604292]: A form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. It is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting. Note=The disease is caused by mutations affecting the gene represented in this entry.
Split-hand/foot malformation 4 (SHFM4) [MIM:605289]: A limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Note=The disease is caused by mutations affecting the gene represented in this entry.
Limb-mammary syndrome (LMS) [MIM:603543]: Characterized by ectrodactyly, cleft palate and mammary-gland abnormalities. Note=The disease is caused by mutations affecting the gene represented in this entry.
Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and
DAPI溶液(即用型10μg/ml )操作步驟Ectodermal dysplasia, Rapp-Hodgkin type (EDRH) [MIM:129400]: A form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. Characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip,
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