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DAPI溶液(1mg/ml)操作步驟*,*,庫(kù)存現(xiàn)貨,無(wú)效果,免費(fèi)退款。公司現(xiàn)貨銷(xiāo)售,量大折扣,咨詢(xún)。詳細(xì)信息如下。
產(chǎn)品名稱(chēng) | 英文名稱(chēng) | 包裝 |
DAPI溶液(1mg/ml)操作步驟 | DAPI solution (1MG/ML) | 可根據(jù)客戶(hù)要求訂制 |
DAPI溶液(1mg/ml)操作步驟產(chǎn)品描述
DAPI溶液(1mg/ml)即 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI),中文名為4’,6-二脒基-2-苯基吲哚。本產(chǎn)品是一種可以穿透細(xì)胞膜的DNA鏈藍(lán)色熒光染料,DAPI染色常用于細(xì)胞凋亡檢測(cè),染色后用熒光顯微鏡觀察或流式細(xì)胞儀檢測(cè)。DAPI也常用于普通的細(xì)胞核染色以及某些特定情況下的雙鏈DNA染色。
產(chǎn)品優(yōu)勢(shì)
本產(chǎn)品為DAPI水溶液,純度≥90%,濃度為1mg/ml。使用時(shí)根據(jù)實(shí)驗(yàn)不同直接將本產(chǎn)品用相應(yīng)溶液稀釋到工作濃度。
儲(chǔ)存條件
−20℃避光保存,有效期至少2年。
DAPI溶液(1mg/ml)操作步驟注意事項(xiàng)?
DAPI對(duì)人體有一定刺激性,請(qǐng)注意適當(dāng)防護(hù)。
熒光染料都存在淬滅的問(wèn)題,建議染色后盡量當(dāng)天完成檢測(cè)。
為減緩熒光淬滅可以使用抗熒光淬滅封片液。
為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
DAPI溶液(1mg/ml)操作步驟分子生物學(xué)領(lǐng)域:
涉及核酸純化、常用分子生物學(xué)試劑、PCR等相關(guān)試劑盒。
分子生物學(xué)試劑:擁有的價(jià)格優(yōu)勢(shì),品質(zhì)可信賴(lài),市場(chǎng)占比龐大。
DAPI溶液(1mg/ml)操作步驟收貨注意事項(xiàng):
當(dāng)您收到貨物后,請(qǐng)仔細(xì)檢查產(chǎn)品的名稱(chēng)、數(shù)量、質(zhì)量規(guī)格是否與您訂購(gòu)產(chǎn)品*。若產(chǎn)生異議,請(qǐng)及時(shí)與我司。按照我司指令及時(shí)將貨物退回,我司*時(shí)間為您安排配送正確貨物。請(qǐng)您務(wù)必保證退還貨物的包裝完整性。
當(dāng)您收到貨物后,若發(fā)現(xiàn)包裝破損,產(chǎn)品泄露等意外情況,請(qǐng)及時(shí)拍照留證,并將照片傳回我司,我司確認(rèn)后,將及時(shí)為您更換新的貨物,原有貨物按照我司指令處理。
我司默認(rèn)貨物簽收24小時(shí)內(nèi)無(wú)反饋,即視為您已經(jīng)認(rèn)同我司到貨情況,未出現(xiàn)包裝破損、貨物錯(cuò)誤等問(wèn)題。
因大多情況貨物是以快遞形式配送,請(qǐng)盡量本人簽收,如由您認(rèn)同的其他人或第三方代為簽收,也認(rèn)同與您本人簽收具有相同的效力,如24小時(shí)內(nèi)無(wú)反饋,默認(rèn)貨物完整正確送達(dá)。
我司所有產(chǎn)品的質(zhì)量追溯期為90天,若有質(zhì)量異議,請(qǐng)務(wù)必在90天內(nèi)提出,超過(guò)90天后,我司默認(rèn)貴處已經(jīng)認(rèn)同該產(chǎn)品的質(zhì)量。
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optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Proliferation Marker
Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA DAPI溶液(1mg/ml)操作步驟synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.
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